Selected article for: "master mix and unprocessed sample"
Author: Arunkumar Arumugam; Season S Wong
Title: The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step
  • Document date: 2020_4_8
    • ID: ka3n9pft_4
    Snippet: To test the extent of PCR inhibition exerted by flu specimens in VTM, we used clinical samples from Discovery Life Sciences, a Biospecimen repository. Between 0.1 µL to 6 µL of the unprocessed samples were directly spiked into the master mix with InfA primers in a 20 µL reaction mix (Fig. 2 ). An extracted template (in Promega Maxwell device) from the same sample was also amplified along with unprocessed samples. Adding more unprocessed sample.....
    Document: To test the extent of PCR inhibition exerted by flu specimens in VTM, we used clinical samples from Discovery Life Sciences, a Biospecimen repository. Between 0.1 µL to 6 µL of the unprocessed samples were directly spiked into the master mix with InfA primers in a 20 µL reaction mix (Fig. 2 ). An extracted template (in Promega Maxwell device) from the same sample was also amplified along with unprocessed samples. Adding more unprocessed samples (up to 6 µL) improved (reduced) the Ct values. The Ct difference between the extracted template (100 µL input and 100 µL eluate) and the unprocessed sample is minimal (29.3 vs. 30.3, respectively).

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