Author: Wang, Wei; Wei, Lei; Yang, Anqi; He, Teng; Yuen, K. Y.; Chen, Cheng; Rao, Zihe
Title: Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9 Cord-id: 5xltdxwk Document date: 2009_4_28
ID: 5xltdxwk
Snippet: Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Ã… resolution and belonged to space group P2(1)2(1)2, with unit-cell para
Document: Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Å resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 Å, α = β = γ = 90° and two molecules per asymmetric unit.
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