Selected article for: "ELISA assay and negative control"

Author: Anastassia K. Pogoutse; Trevor F. Moraes
Title: Transferrin binding protein B and Transferrin binding protein A 2 expand the transferrin recognition range of Histophilus somni
  • Document date: 2019_8_9
  • ID: 2chmny8w_2
    Snippet: In order to see if we could recapitulate the results of the previously published study, we 125 performed a binding experiment with whole fixed cells of H. somni strain H191. We performed a 126 competitive assay known as a displacement ELISA, and looked at binding of whole fixed cells of 127 H. somni to bovine, ovine, caprine, and porcine transferrins. Cattle, sheep, and goats are known 128 hosts of H. somni while swine are not, and so porcine tra.....
    Document: In order to see if we could recapitulate the results of the previously published study, we 125 performed a binding experiment with whole fixed cells of H. somni strain H191. We performed a 126 competitive assay known as a displacement ELISA, and looked at binding of whole fixed cells of 127 H. somni to bovine, ovine, caprine, and porcine transferrins. Cattle, sheep, and goats are known 128 hosts of H. somni while swine are not, and so porcine transferrin was used as a negative control 129 ( Figure 1A ). H. somni bound bovine transferrin, as expected, but also displayed some affinity for 130 ovine transferrin. There was no statistically significant binding to the caprine or porcine 131 transferrins ( Figure 1B) . 132

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