Author: Anastassia K. Pogoutse; Trevor F. Moraes
Title: Transferrin binding protein B and Transferrin binding protein A 2 expand the transferrin recognition range of Histophilus somni Document date: 2019_8_9
ID: 2chmny8w_27
Snippet: The dialyzed 70% ammonium sulfate fraction was further purified using anion exchange 369 chromatography using an in-house packed 25 mL Q-Sepharose column. After multiple rounds of 370 chromatography, primarily done to separate transferrin from albumin, the protein was 371 concentrated and size exclusion chromatography using an S75 column (GE) was used for buffer 372 exchange into PBS. The protein was concentrated further (if necessary), aliquoted.....
Document: The dialyzed 70% ammonium sulfate fraction was further purified using anion exchange 369 chromatography using an in-house packed 25 mL Q-Sepharose column. After multiple rounds of 370 chromatography, primarily done to separate transferrin from albumin, the protein was 371 concentrated and size exclusion chromatography using an S75 column (GE) was used for buffer 372 exchange into PBS. The protein was concentrated further (if necessary), aliquoted, flash-frozen, 373 and stored at -80 C. 374
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