Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer Document date: 2020_4_8
ID: jpb9a8c4_11
Snippet: 1. 472.75 g of GITC is brought into solution initially by adding 400 ml of 0.1 M Tris HCl pH 7.6. This will require heating in a 65°C water bath and some shaking of the vessel (but with lid well secured). In our hands, once fully dissolved the volume of the solution was 600 ml. 2. Make up to 750 ml with 0.1 M Tris HCl pH 7.6. 3. Add 50 ml of 0.5 M EDTA, mix. 4. Add 30 ml Triton-X-100, mix 5. Volume made up to 1 L with 0.04 % (w/v) bromophenol bl.....
Document: 1. 472.75 g of GITC is brought into solution initially by adding 400 ml of 0.1 M Tris HCl pH 7.6. This will require heating in a 65°C water bath and some shaking of the vessel (but with lid well secured). In our hands, once fully dissolved the volume of the solution was 600 ml. 2. Make up to 750 ml with 0.1 M Tris HCl pH 7.6. 3. Add 50 ml of 0.5 M EDTA, mix. 4. Add 30 ml Triton-X-100, mix 5. Volume made up to 1 L with 0.04 % (w/v) bromophenol blue (DEPC-treated water can be used instead)
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