Selected article for: "diagnostic laboratory and International license"
Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer
  • Document date: 2020_4_8
    • ID: jpb9a8c4_23
    Snippet: Heeding Dr Michael J. Ryan's advice "Perfection is the enemy of the good when it comes to emergency management", we do not want to delay in communicating our findings to the wider world. Further variations of the 4 M GITC lysis buffer . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 formulat.....
    Document: Heeding Dr Michael J. Ryan's advice "Perfection is the enemy of the good when it comes to emergency management", we do not want to delay in communicating our findings to the wider world. Further variations of the 4 M GITC lysis buffer . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 formulation can be tested further, in particular formulations with graded increases in % w/v of Triton X-100 could be compared. Work is ongoing to test if variations in the molarity of Tris-HCl affect lysis buffer performance. Of highest priority is to get the message out that this 4 M GITC lysis buffer works well. In addition we observed that Qiagen's RLT lysis buffer from RNeasy kits worked very well with the systems and processes in place in the diagnostic laboratory at Cork University Hospital.

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