Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM

Chuang Liu; Yang Yang; Yuanzhu Gao; Chenguang Shen; Bin Ju; Congcong Liu; Xian Tang; Jinli Wei; Xiaomin Ma; Weilong Liu; Shuman Xu; Yingxia Liu; Jing Yuan; Jing Wu; Zheng Liu; Zheng Zhang; Peiyi Wang; Lei Liu

The amount of 3µl purified samples were applied to glow-discharged grid with a continuous carbon layer (Electron microscopy China, China) for 1min. The grid was blotted by filter paper to absorb the excrescent sample. The grid was then stained with 3%(w/w) phosphotungstic acid for 1min. Redundant liquid was absorbed using filter paper. The grid was transferred to a Talos 120C transmission electron microscope (Thermo Fisher Scientific) performed at the 120 kV in low dose mode and imaged with a Ceta 16M CMOS detector (Thermo Fisher Scientific). Data were collected at a nominal magnification of 45000 x, corresponding to a pixel size of 3.5 Å/pixel.