Author: Chuang Liu; Yang Yang; Yuanzhu Gao; Chenguang Shen; Bin Ju; Congcong Liu; Xian Tang; Jinli Wei; Xiaomin Ma; Weilong Liu; Shuman Xu; Yingxia Liu; Jing Yuan; Jing Wu; Zheng Liu; Zheng Zhang; Peiyi Wang; Lei Liu
Title: Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM Document date: 2020_3_5
ID: cr22vp8b_8
Snippet: The bronchoalveolar lavage fluid (BALF) sample was collected and subjected to nextgeneration sequencing and virus isolation was performed in the BSL-3 (Biosafety level 3) laboratory. Typical cytopathic effects (CPE) were observed at 4 days post inoculation (dpi) in Vero cell, including cell rounding, shrinkage, lysis, and detachment throughout the cell monolayers ( Figure 1A ). Viral RNAs could be detected in the cell culture supernatant using a .....
Document: The bronchoalveolar lavage fluid (BALF) sample was collected and subjected to nextgeneration sequencing and virus isolation was performed in the BSL-3 (Biosafety level 3) laboratory. Typical cytopathic effects (CPE) were observed at 4 days post inoculation (dpi) in Vero cell, including cell rounding, shrinkage, lysis, and detachment throughout the cell monolayers ( Figure 1A ). Viral RNAs could be detected in the cell culture supernatant using a CFDA approved commercial kit with low Ct values ( Figure 1B ).
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