Author: Chuang Liu; Yang Yang; Yuanzhu Gao; Chenguang Shen; Bin Ju; Congcong Liu; Xian Tang; Jinli Wei; Xiaomin Ma; Weilong Liu; Shuman Xu; Yingxia Liu; Jing Yuan; Jing Wu; Zheng Liu; Zheng Zhang; Peiyi Wang; Lei Liu
Title: Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM Document date: 2020_3_5
ID: cr22vp8b_8
Snippet: The bronchoalveolar lavage fluid (BALF) sample was collected and subjected to nextgeneration sequencing and virus isolation was performed in the BSL-3 (Biosafety level 3) laboratory. Typical cytopathic effects (CPE) were observed at 4 days post inoculation (dpi) in Vero cell, including cell rounding, shrinkage, lysis, and detachment throughout the cell monolayers ( Figure 1A ). Viral RNAs could be detected in the cell culture supernatant using a .....
Document: The bronchoalveolar lavage fluid (BALF) sample was collected and subjected to nextgeneration sequencing and virus isolation was performed in the BSL-3 (Biosafety level 3) laboratory. Typical cytopathic effects (CPE) were observed at 4 days post inoculation (dpi) in Vero cell, including cell rounding, shrinkage, lysis, and detachment throughout the cell monolayers ( Figure 1A ). Viral RNAs could be detected in the cell culture supernatant using a CFDA approved commercial kit with low Ct values ( Figure 1B ).
Search related documents:
Co phrase search for related documents- BALF sample and cell culture: 1
- BALF sample and cell monolayer: 1
- BALF sample and lavage fluid: 1, 2, 3
- Biosafety level and cell culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26
- Biosafety level and cell culture supernatant: 1, 2, 3
- Biosafety level and commercial kit: 1, 2, 3
- Biosafety level and Ct value: 1, 2
- Biosafety level and cytopathic effect: 1, 2, 3, 4, 5, 6, 7, 8
- Biosafety level and dpi post inoculation: 1
- Biosafety level and post inoculation: 1, 2
- Biosafety level and Vero cell: 1, 2
- Biosafety level and viral rna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- Biosafety level and virus isolation: 1, 2
- bronchoalveolar lavage fluid and cell culture: 1, 2, 3, 4, 5
- bronchoalveolar lavage fluid and commercial kit: 1
- bronchoalveolar lavage fluid and dpi post inoculation: 1
- bronchoalveolar lavage fluid and lavage fluid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78
- bronchoalveolar lavage fluid and post inoculation: 1, 2
- bronchoalveolar lavage fluid and viral rna: 1, 2, 3, 4, 5, 6, 7, 8, 9
Co phrase search for related documents, hyperlinks ordered by date