Lack of evidence for infectious SARS-CoV-2 in feces and sewage

Albert, Sandra; Ruíz, Alba; Pemán, Javier; Salavert, Miguel; Domingo-Calap, Pilar

The SARS-CoV-2 can be excreted in feces and can reach sewage systems. Determining the presence of infective viral particles in feces and sewage is necessary to take adequate control measures and to elucidate new routes of transmission. Here, we have developed a sample concentration methodology that allows us to maintain viral infectivity. Feces of COVID-19 patients and wastewater samples have been analyzed both by molecular methods and cell culture. Our results show no evidence of infective viral particles, suggesting that fecal–oral transmission is not a primary route. However, larger-scale efforts are needed, especially with the emergence of new viral variants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10096-021-04304-4.