A hidden gene in astroviruses encodes a cell-permeabilizing protein involved in virus release

Valeria Lulla; Andrew E. Firth

To normalize for library size, reads per million mapped reads (RPM) values were calculated using the sum of positive-sense virus and host RefSeq mRNA reads as the denominator. A +12 nt offset was applied to the RPF 5′ end positions to give the approximate ribosomal P-site positions. To calculate the phasing and length distributions of host and virus RPFs, only RPFs whose 5′ end (+12 nt offset) mapped between the 13th nucleotide from the beginning and the 18th nucleotide from the end of coding sequences (ORF1a, ORF1b and ORF2 for HAstV;