Author: Gerna, Giuseppe; Campanini, Giulia; Rovida, Francesca; Percivalle, Elena; Sarasini, Antonella; Marchi, Antonietta; Baldanti, Fausto
Title: Genetic variability of human coronavirus OC43â€, 229Eâ€, and NL63â€like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patients Cord-id: cmjnb0al Document date: 2006_5_23
ID: cmjnb0al
Snippet: In the winter–spring seasons 2003–2004 and 2004–2005, 47 (5.7%) patients with acute respiratory infection associated with human coronavirus (hCoV) 229Eâ€, NL63â€, and OC43â€like strains were identified among 823 (597 immunocompetent and 226 immunocompromised) patients admitted to hospital with acute respiratory syndromes. Viral infections were diagnosed by either immunological (monoclonal antibodies) or molecular (RTâ€PCR) methods. Each of two sets of primer pairs developed for detecti
Document: In the winter–spring seasons 2003–2004 and 2004–2005, 47 (5.7%) patients with acute respiratory infection associated with human coronavirus (hCoV) 229Eâ€, NL63â€, and OC43â€like strains were identified among 823 (597 immunocompetent and 226 immunocompromised) patients admitted to hospital with acute respiratory syndromes. Viral infections were diagnosed by either immunological (monoclonal antibodies) or molecular (RTâ€PCR) methods. Each of two sets of primer pairs developed for detection of all CoVs (panCoV) failed to detect 15 of the 53 (28.3%) hCoV strains identified. On the other hand, all hCoV strains could be detected by using typeâ€specific primers targeting genes 1ab and N. The HuHâ€7 cell line was found to be susceptible to isolation and identification of OC43†and 229Eâ€like strains. Overall, hCoV infection was caused by OC43â€like, 229Eâ€like, and NL63â€like strains in 25 (53.2%), 10 (21.3%), and 9 (19.1%) patients, respectively. In addition, three patients (6.4%) were infected by untypeable hCoV strains. NL63â€like strains were not found to circulate in 2003–2004, and 229Eâ€like strains did not circulate in 2004–2005, while OC43â€like strains were detected in both seasons. The monthly distribution reached a peak during January through March. Lower predominated over upper respiratory tract infections in each age group. In addition, hCoV infections interested only immunocompetent infants and young children during the first year of life, while all adults were immunocompromised patients. Coinfections of hCoVs and other respiratory viruses (mostly interesting the first year of life) were observed in 14 of the 47 (29.8%) patients and were associated with severe respiratory syndromes more frequently than hCoV single infections (P = 0.002). In conclusion, the use of multiple primer sets targeting different genes is recommended for diagnosis of all types of hCoV infection. In addition, the detection of still untypeable hCoV strains suggests that the number of hCoVs involved in human pathology might further increase. Finally, hCoVs should be screened routinely for in both infants and immunocompromised patients with acute respiratory infection. J. Med. Virol. 78:938–949, 2006. © 2006 Wileyâ€Liss, Inc.
Search related documents:
Co phrase search for related documents- acute respiratory infection and low respiratory: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute respiratory infection and low respiratory tract: 1, 2, 3, 4, 5
- acute respiratory infection and low respiratory tract infection: 1, 2
- acute respiratory syndrome and long term short term: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute respiratory syndrome and low respiratory: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute respiratory syndrome and low respiratory tract: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- acute respiratory tract infection and low respiratory: 1, 2, 3, 4, 5, 6
- acute respiratory tract infection and low respiratory tract: 1, 2, 3
- acute respiratory tract infection and low respiratory tract infection: 1, 2
- long term short term and low respiratory: 1
Co phrase search for related documents, hyperlinks ordered by date