A novel antiviral formulation inhibits a range of enveloped viruses.

Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird

The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.03.29.009464 doi: bioRxiv preprint bit a defined area of skin (upper side of the left foot), anesthetized mice were placed for up to 10 min into a mosquito cage containing A. aegypti mosquitoes (locally bred colony derived from the Liverpool strain). Biting was restricted to a defined area of the left foot by covering all other mouse skin with an impenetrable barrier. Viral RNA and host gene transcripts in tissues were quantified by reverse transcription qPCR, and infectious virus was quantified by end point titration, as described previously. Tissue generated up to 100 ug of total RNA, of which 1 ug of RNA was used to create cDNA, of which 1% was used per qPCR assay. qPCR primers for SFV amplified a section of E1 and primers for ZIKV amplified a section of the env gene. For SFV and ZIKV, qPCR assays measured the sum value of both genome and subgenomic RNA (Bryden et al., 2020) . Each result represents the median of three or four technical replicates of one biological replicate. For plaque assay, viral stocks and biological samples were serially diluted, and each dilution was assayed in duplicate the author/funder. All rights reserved. No reuse allowed without permission.