Author: Jennifer N Rauch; Eric Valois; Sabrina C Solley; Friederike Braig; Ryan S Lach; Naomi J Baxter; Kenneth S Kosik; Carolina Arias; Diego Acosta-Alvear; Maxwell Z Wilson
Title: A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material Document date: 2020_4_21
ID: hy1xrw6q_3
Snippet: A critical hurdle to deploying massively widespread, recurrent testing is the availability of reagents and specialized equipment. Suggested solutions to make sample collection scalable include laboratory-made Viral Transport Media and 3Dprinted swabs and bypassing biochemical RNA extraction through heat and chemical extraction techniques [11] [12] [13] [14] . Yet, there have been few end-toend solutions to SARS-CoV-2 testing that fulfill the requ.....
Document: A critical hurdle to deploying massively widespread, recurrent testing is the availability of reagents and specialized equipment. Suggested solutions to make sample collection scalable include laboratory-made Viral Transport Media and 3Dprinted swabs and bypassing biochemical RNA extraction through heat and chemical extraction techniques [11] [12] [13] [14] . Yet, there have been few end-toend solutions to SARS-CoV-2 testing that fulfill the requirements of being immediately scalable and low-cost, without sacrificing sensitivity. Here, we focused on developing a CRISPR-based SARS-CoV-2 RNA detection method that is low cost, highly sensitive, and easy to deploy at sites with minimal infrastructure.
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