Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_15
Snippet: Then, the pcMNPs-RNA complex was collected magnetically for 1 min and the supernatant was discarded. Afterwards, the complex was washed once or twice with 400 μL washing buffer (75% ethanol v/v). The purified nucleic acids were released from MNPs by incubating the complex in 50 μL of TE buffer (10 mM Tris-HCl, pH 8.0), vortexed at 55 o C bath for 5 min, and 15 μL of supernatant was transferred to subsequent RT-PCR reaction......
Document: Then, the pcMNPs-RNA complex was collected magnetically for 1 min and the supernatant was discarded. Afterwards, the complex was washed once or twice with 400 μL washing buffer (75% ethanol v/v). The purified nucleic acids were released from MNPs by incubating the complex in 50 μL of TE buffer (10 mM Tris-HCl, pH 8.0), vortexed at 55 o C bath for 5 min, and 15 μL of supernatant was transferred to subsequent RT-PCR reaction.
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