A speed-fidelity trade-off determines the mutation rate and virulence of an RNA virus

William Fitzsimmons; Robert J. Woods; John T. McCrone; Andrew Woodman; Jamie J. Arnold; Madhumita Yennawar; Richard Evans; Craig E. Cameron; Adam S. Lauring

although there were 7 total among 30 plaques (Table S1 ). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/309880 doi: bioRxiv preprint 18 MOI of 0.5 (6 x 10 5 TCID 50 units) in 1ml of media for one hour with occasional rocking. After one 442 hour, the inoculum was aspirated, the cells were washed twice with PBS, and 2ml of fresh 443 growth media applied. For the first 15 passages, WT and 3D G64S viruses were harvested at 4 444 and 4.5 hours, respectively. For passages 16-50, WT and 3D G64S viruses were harvested at 3.5 445 and 4 hours, respectively. Control populations were infected in the same manner except that 446 viruses were harvested at 24 hours post infection. There were (5) r-selected WT lineages, (5) r-447 selected 3D G64S lineages, (5) control WT lineages, and (5) control 3D G64S lineages. Viruses were 448 titered at every 5 th passage to maintain an MOI of approximately 0.5.