Author: Cara E. Brook; Mike Boots; Kartik Chandran; Andrew P. Dobson; Christian Drosten; Andrea L. Graham; Bryan T. Grenfell; Marcel A. Müller; Melinda Ng; Lin-Fa Wang; Anieke van Leeuwen
Title: Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence Document date: 2019_7_8
ID: 683qcgd9_11
Snippet: Green, then cycled on a QuantStudio3 Real-Time PCR machine under the following conditions: 533 initial denaturation at 94ËšC for 2 min followed by 40 cycles of: denaturation at 95ËšC (5 sec), 534 annealing at 58ËšC (15 sec), and extension at 72ËšC (10 sec). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/696195 doi: bioRxiv preprint 30 for each of the three cell lines (Figure.....
Document: Green, then cycled on a QuantStudio3 Real-Time PCR machine under the following conditions: 533 initial denaturation at 94ËšC for 2 min followed by 40 cycles of: denaturation at 95ËšC (5 sec), 534 annealing at 58ËšC (15 sec), and extension at 72ËšC (10 sec). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/696195 doi: bioRxiv preprint 30 for each of the three cell lines (Figure 1-figure supplement 7) . This yielded a natural mortality 630 rate, , corresponding to a lifespan of approximately 121, 191, and 84 hours, respectively, for 631
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