Author: Linlin Zhang; Daizong Lin; Yuri Kusov; Yong Nian; Qingjun Ma; Jiang Wang; Albrecht von Brunn; Pieter Leyssen; Kristina Lanko; Johan Neyts; Adriaan de Wilde; Eric J. Snijder; Hong Liu; Rolf Hilgenfeld
Title: Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication Document date: 2020_2_10
ID: 7n8p9okf_62
Snippet: Subgenomic replicons of CVB3 (pT7-CVB3-FLuc 34 ) and EV-A71 (pT7-EV71-RLuc) harbouring T7-controlled complete viral genomes, in which the P1 capsid-coding sequence was replaced by the Firefly (Photinus pyralis) or Renilla (Renilla renifor) luciferase gene, were generously provided by F. van Kuppeveld and B. Zhang, respectively. To prepare CVB3 and EV-A71 replicon RNA transcripts, plasmid DNAs were linearized by digestion with SalI or HindIII (New.....
Document: Subgenomic replicons of CVB3 (pT7-CVB3-FLuc 34 ) and EV-A71 (pT7-EV71-RLuc) harbouring T7-controlled complete viral genomes, in which the P1 capsid-coding sequence was replaced by the Firefly (Photinus pyralis) or Renilla (Renilla renifor) luciferase gene, were generously provided by F. van Kuppeveld and B. Zhang, respectively. To prepare CVB3 and EV-A71 replicon RNA transcripts, plasmid DNAs were linearized by digestion with SalI or HindIII (New England Biolabs), respectively. Copy RNA transcripts were synthesized in vitro using linearized DNA templates, T7 RNA polymerase, and the T7 RiboMaxâ„¢ Large-Scale RNA Production System (Promega) according to the manufacturer's recommendations.
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