An interaction network of RNA-binding proteins involved in Drosophila oogenesis

Prashali Bansal; Johannes Madlung; Kristina Schaaf; Boris Macek; Fulvia Bono

Consistent with the well-documented role of RNA helicases in oogenesis and fertility (87, (123) (124) (125) (126) , we identified two putative DEAD-box helicases, CG10077 and Mahe (Maheshvara). While CG10077 is an ortholog of human DDX5, Mahe is an evolutionary conserved regulator of Notch signaling (127) . We could recapitulate the interaction of Mahe with both Hrp48 and Glo, and CG10077 with Glo in our co-IP assay. mRNAs. Integrating isolated complexes into interaction networks will further enable functional insights into poorly characterized proteins. Given the evolutionary conservation of several RBPs, our study provides a framework to transfer information to other systems, enhancing our understanding of regulation of RBPs and their diverse roles in developmental processes. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.08.899146 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.08.899146 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.08.899146 doi: bioRxiv preprint denotes the number of protein groups plotted and "r" denotes the Pearson correlation coefficient. Each identified protein is represented as a dot in light grey; each bait is highlighted in green; significantly enriched proteins are highlighted in pink; known interactants are highlighted in blue; background binders are highlighted in dark grey; empty circle represents control. whereas for HA-Flag-tagged protein, 3.4% of the input and 90% of the eluates were analyzed. In each panel, cell lysates were treated with RNases before immunoprecipitation. Novel interactions are highlighted in red. Significantly enriched proteins in labeled MS data (ratio>2 in both replicates)