Selected article for: "copy number and dna molecule"
Author: Gustavo Barcelos Barra; Ticiane Henriques Santa Rita; Pedro Goes Mesquita; Rafael Henriques Jacomo; Lidia Freire Abdalla Nery
Title: Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow
  • Document date: 2020_3_10
    • ID: kv77pw7y_15
    Snippet: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.03.07.20032326 doi: medRxiv preprint A synthetic dsDNA molecule (Integrated DNA Technologies, Coralville, USA) comprising of the concatenation of all six viral assays target sequences in the SARS-CoV-2 genome was in vitro transcribed into its RNA form. This SARS-CoV-2 diagnostic synthetic RNA was quantified and a previously known copy number.....
    Document: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.03.07.20032326 doi: medRxiv preprint A synthetic dsDNA molecule (Integrated DNA Technologies, Coralville, USA) comprising of the concatenation of all six viral assays target sequences in the SARS-CoV-2 genome was in vitro transcribed into its RNA form. This SARS-CoV-2 diagnostic synthetic RNA was quantified and a previously known copy number solution was spiked into negative swabs to construct positive samples as similar as possible from real clinical samples. These constructed samples were submitted to the automated workflow for assays that passed in the analytical specificity evaluation (N1, E, and RdRP).

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