Selected article for: "assay detection and detection limit"
Author: Gustavo Barcelos Barra; Ticiane Henriques Santa Rita; Pedro Goes Mesquita; Rafael Henriques Jacomo; Lidia Freire Abdalla Nery
Title: Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow
  • Document date: 2020_3_10
    • ID: kv77pw7y_16
    Snippet: Limiting dilution of 1:2 (from 2.64x10 4 to 4.04x10 -1 copies/reaction) of the synthetic SARS-CoV-2 diagnostic RNA was tested in order to evaluate the assays' limit of detection. Probit regression analysis returned the limit of detection of 21 (95% CI 16.5 -31.1) copies/reaction for N1, 141 (95% CI 109 -207) copies/reaction for E and 350 (95% CI 281 -508) copies/reaction for RdRP (Figure 1, B ). E and RdRP assay primer/probe concentrations were m.....
    Document: Limiting dilution of 1:2 (from 2.64x10 4 to 4.04x10 -1 copies/reaction) of the synthetic SARS-CoV-2 diagnostic RNA was tested in order to evaluate the assays' limit of detection. Probit regression analysis returned the limit of detection of 21 (95% CI 16.5 -31.1) copies/reaction for N1, 141 (95% CI 109 -207) copies/reaction for E and 350 (95% CI 281 -508) copies/reaction for RdRP (Figure 1, B ). E and RdRP assay primer/probe concentrations were modified and limits of detection changed to 457 (95%CI 382 -598) and 33.7 (95% CI 27.6 -46.8) copies/reaction, respectively. Primers/probe concentration optimization did not interfere with specificity of the assays (20 healthy volunteers samples tested).

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