Selected article for: "detection limit and probit analysis"
Author: Gustavo Barcelos Barra; Ticiane Henriques Santa Rita; Pedro Goes Mesquita; Rafael Henriques Jacomo; Lidia Freire Abdalla Nery
Title: Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow
  • Document date: 2020_3_10
    • ID: kv77pw7y_16
    Snippet: Limiting dilution of 1:2 (from 2.64x10 4 to 4.04x10 -1 copies/reaction) of the synthetic SARS-CoV-2 diagnostic RNA was tested in order to evaluate the assays' limit of detection. Probit regression analysis returned the limit of detection of 21 (95% CI 16.5 -31.1) copies/reaction for N1, 141 (95% CI 109 -207) copies/reaction for E and 350 (95% CI 281 -508) copies/reaction for RdRP (Figure 1, B ). E and RdRP assay primer/probe concentrations were m.....
    Document: Limiting dilution of 1:2 (from 2.64x10 4 to 4.04x10 -1 copies/reaction) of the synthetic SARS-CoV-2 diagnostic RNA was tested in order to evaluate the assays' limit of detection. Probit regression analysis returned the limit of detection of 21 (95% CI 16.5 -31.1) copies/reaction for N1, 141 (95% CI 109 -207) copies/reaction for E and 350 (95% CI 281 -508) copies/reaction for RdRP (Figure 1, B ). E and RdRP assay primer/probe concentrations were modified and limits of detection changed to 457 (95%CI 382 -598) and 33.7 (95% CI 27.6 -46.8) copies/reaction, respectively. Primers/probe concentration optimization did not interfere with specificity of the assays (20 healthy volunteers samples tested).

    Search related documents:
    Co phrase search for related documents
    • assay detection limit and dilution limit: 1, 2, 3
    • assay detection limit and primer probe: 1, 2, 3, 4, 5
    • assay detection limit evaluate and detection limit: 1
    • assay specificity and copy reaction: 1, 2, 3, 4
    • assay specificity and detection limit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
    • assay specificity and dilution limit: 1
    • assay specificity and primer probe: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • assay specificity and primer probe concentration: 1
    • assay specificity and probit regression: 1
    • assay specificity and probit regression analysis: 1
    • assay specificity and RdRP reaction: 1, 2
    • copy N1 reaction and N1 reaction: 1
    • copy N1 reaction and respectively copy reaction: 1
    • copy reaction and detection limit: 1, 2, 3, 4, 5, 6