Author: Chantal B.F. Vogels; Anderson F. Brito; Anne Louise Wyllie; Joseph R Fauver; Isabel M. Ott; Chaney C. Kalinich; Mary E. Petrone; Marie-Louise Landry; Ellen F. Foxman; Nathan D. Grubaugh
Title: Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays Document date: 2020_4_1
ID: 6mdimxnk_16
Snippet: To investigate if the background cross-reactivity with pre-COVID-19 nasopharyngeal swabs that we observed for the US CDC 2019-nCoV_N2 primer-probe set ( Fig. 3 ) would impact the testing outcomes of the US CDC assay, we compared 2019-nCoV_N1 and N2 results from 172 clinical samples taken during the COVID-19 pandemic ( Fig. 4 ). We found that N1 was typically more sensitive, yielding lower Ct values from positive samples, and that background cross.....
Document: To investigate if the background cross-reactivity with pre-COVID-19 nasopharyngeal swabs that we observed for the US CDC 2019-nCoV_N2 primer-probe set ( Fig. 3 ) would impact the testing outcomes of the US CDC assay, we compared 2019-nCoV_N1 and N2 results from 172 clinical samples taken during the COVID-19 pandemic ( Fig. 4 ). We found that N1 was typically more sensitive, yielding lower Ct values from positive samples, and that background cross-reactivity with N2 did not yield an abundance of inconclusive test results.
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