Author: Andrew C Nelson; Benjamin Auch; Matthew Schomaker; Daryl M Gohl; Patrick Grady; Darrell Johnson; Robyn Kincaid; Kylene E Karnuth; Jerry Daniel; Jessica K Fiege; Elizabeth J Fay; Tyler Bold; Ryan A Langlois; Kenneth B Beckman; Sophia Yohe
Title: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods Document date: 2020_4_5
ID: ec3egn8o_6
Snippet: Samples were tracked in a Laboratory Information Management System (LIMS) throughout the process from accessioning through to data release. Container barcodes used for tracing did not include any PHI. At initial scanning into the LIMS, each unique sample swab barcode was associated with a unique 0.5-ml 2D-barcoded tube (Micronic), into which the extracted RNA was eluted at completion of extraction. Racks of these 2D barcoded tubes from extraction.....
Document: Samples were tracked in a Laboratory Information Management System (LIMS) throughout the process from accessioning through to data release. Container barcodes used for tracing did not include any PHI. At initial scanning into the LIMS, each unique sample swab barcode was associated with a unique 0.5-ml 2D-barcoded tube (Micronic), into which the extracted RNA was eluted at completion of extraction. Racks of these 2D barcoded tubes from extraction batches were arranged into 96-well run-plate layouts, which were bottom-scanned to confirm well location into the LIMS, which then generated PCR run plate layouts, ingested the RT-PCR result files for inspection and interpretation by pathologists, and generated report file outputs for import into laboratory information and electronic medical records systems.
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