Arenaviridae exoribonuclease presents genomic RNA edition capacity

Elsie Yekwa; Chutima Aphibanthammakit; Xavier Carnec; Bruno Coutard; Caroline Picard; Bruno Canard; Sylvain Baize; François Ferron

To study the effect of divalent metal binding on the stability of NP-exo MOPV, we measured the change in melting temperature (Tm) by a Thermal Shift Assay (TSA) in the presence of 5 mM of several metal ions. NP-exo MOPV without metal ions has a Tm of 49.4 °C. Positive Tm shifts are observed in the presence of MnCl 2 (+16.5 °C), MgCl 2 (+4.9 °C), CaCl 2 (+7.5 °C) and a slight negative shift in ZnCl 2 (-2.2 °C) (Fig 2) . Simultaneously we compared the effect of these ions on the stability of NP-exo LCMV (Fig 2) . The Tm value for NP-exo LCMV without metal ions is 50. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/541698 doi: bioRxiv preprint stability in all ExoNs. It is also worth noting that CaCl 2 which inhibits the 3'-5' ExoN activity is a better stabilizer than MgCl 2 .