Author: Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou
Title: Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy Document date: 2020_2_27
ID: egntml7e_25
Snippet: The lentivirus VDR-KD and NC-KD was diluted to a total volume of 200 μl containing 4 × 10 7 TU. BALB/c mice were transfected through tail intravenous injection as reported (Morizono et al., 2005) . One week later, mice were sacrificed and spleen tissues were collect to detect the infection efficiency. Further CLP modeling and AS treatment were employed in transfected mice, and their tissues were collected for the cytokines and western bolting a.....
Document: The lentivirus VDR-KD and NC-KD was diluted to a total volume of 200 μl containing 4 × 10 7 TU. BALB/c mice were transfected through tail intravenous injection as reported (Morizono et al., 2005) . One week later, mice were sacrificed and spleen tissues were collect to detect the infection efficiency. Further CLP modeling and AS treatment were employed in transfected mice, and their tissues were collected for the cytokines and western bolting assays.
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