Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_56
Snippet: A spinning disc confocal microscope (Zeiss Axio Observer Z1 with Yokagawa CSU-X1M) was used to image GUVs and tethered vesicles. Laser wavelengths of 488 and 561 nm were used for excitation. Emission filters were centered at 525 nm with a 50 nm width, and 629 nm with a 62 nm width. A triple-pass dichroic mirror was used: 405/488/561 nm. The microscope objective was a Plan-Apochromat 100x, 1.4 numerical aperture oil immersion objective. Images wer.....
Document: A spinning disc confocal microscope (Zeiss Axio Observer Z1 with Yokagawa CSU-X1M) was used to image GUVs and tethered vesicles. Laser wavelengths of 488 and 561 nm were used for excitation. Emission filters were centered at 525 nm with a 50 nm width, and 629 nm with a 62 nm width. A triple-pass dichroic mirror was used: 405/488/561 nm. The microscope objective was a Plan-Apochromat 100x, 1.4 numerical aperture oil immersion objective. Images were collected on a cooled (-70 °C) EMCCD iXon3 897 camera (Andor Technology; Belfast, UK).
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