Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_85
Snippet: Cell culture and transfection BFP-CLC RPE cells were grown in 1:1 F12:DMEM supplemented with 10% FBS, 20 mM HEPES, Pen/Strep/L-glutamine (100 units/ml, 100 μg/ml, 300 μg/ml respectively) and incubated at 37°C with 5% CO 2 . Cells were seeded onto acid washed coverslips at a density of 5 x 10 4 cells per coverslip for 24 hours before transfection with 1-2 µg of plasmid DNA using 3 µL Fugene transfection reagent per µg of DNA (Promega, Madiso.....
Document: Cell culture and transfection BFP-CLC RPE cells were grown in 1:1 F12:DMEM supplemented with 10% FBS, 20 mM HEPES, Pen/Strep/L-glutamine (100 units/ml, 100 μg/ml, 300 μg/ml respectively) and incubated at 37°C with 5% CO 2 . Cells were seeded onto acid washed coverslips at a density of 5 x 10 4 cells per coverslip for 24 hours before transfection with 1-2 µg of plasmid DNA using 3 µL Fugene transfection reagent per µg of DNA (Promega, Madison, WI, All rights reserved. No reuse allowed without permission.
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