Author: Joshua A. Hayward; Mary Tachedjian; Adam Johnson; Tamsin B. Gordon; Jie Cui; Glenn A. Marsh; Michelle L. Baker; Lin-Fa Wang; Gilda Tachedjian
Title: Bats Possess Unique Variants of the Antiviral Restriction Factor Tetherin Document date: 2020_4_9
ID: eksi9yia_59
Snippet: All PCR amplification assays were performed using the Roche FastStart High Fidelity PCR system (Cat # 04738292001) with an annealing temperature gradient of 54°C to 64°C in 2°C increments. Each reaction was made up to a total of 20 μl, containing 1 unit of polymerase, 2 ng of total cDNA, and 8 pmol of each primer. All other parameters for PCR amplification were performed according to the manufacturer's protocol......
Document: All PCR amplification assays were performed using the Roche FastStart High Fidelity PCR system (Cat # 04738292001) with an annealing temperature gradient of 54°C to 64°C in 2°C increments. Each reaction was made up to a total of 20 μl, containing 1 unit of polymerase, 2 ng of total cDNA, and 8 pmol of each primer. All other parameters for PCR amplification were performed according to the manufacturer's protocol.
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