Bioinformatics Study on Structural Protein of Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-Cov-2) For Better Understanding the Vaccine Development

Sumaira Gulzar; Saqib Hussain

The physical parameters (Table 2) show that Surface protein contain highest amino acids (1273), negatively charged residues (110) positively charged residues (103), EC (148960) and molecular weight (141178.47). Higher molecular weight of the surface glycoprotein suggests that its tertiary structure may contain strong amino acid side chains. All three Proteins are basic in nature, excluding surface glycoprotein, as the isoelectric point value is more than 9.The . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.21.053199 doi: bioRxiv preprint instability index value greater than 40 is considered unstable (Lagerstrom et al.,) This is derived from the Instability index surface, membrane and envelop glycoprotein is stable and nucleocapsid phosphoprotein is highly unstable .the range of aliphatic index from 33.01 -55.09 that suggests a tendency to be sensitive to a wide range of temperatures (Ikai ,1980) The SWISS-MODEL system was used for protein structure homology modelling and alignments for all structural proteins of SARS COV 2. First, the right protein template structures in PDB were selected using the given measures: should high reporting of the template (i.e. > 60% target associated with the template) and sequence uniqueness > 35%. Instead, as an initial criterion, we used the GMQE and QMEAN4 scoring method to distinguish well from poor ones . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.21.053199 doi: bioRxiv preprint