Author: Jennifer A. Doudna
Title: Blueprint for a Pop-up SARS-CoV-2 Testing Lab Document date: 2020_4_12
ID: modtthxx_75
Snippet: In the same plate, we included known positive and negative samples taken from leftover samples processed by a local clinical testing facility. These samples were provided to us in the sample collection medium that was used at the facility (universal transport medium) diluted 1:1 with 2x DNA/RNA Shield. To prepare these samples for our workflow, we further diluted them in our sample collection medium to 450uL before performing RNA extraction (see .....
Document: In the same plate, we included known positive and negative samples taken from leftover samples processed by a local clinical testing facility. These samples were provided to us in the sample collection medium that was used at the facility (universal transport medium) diluted 1:1 with 2x DNA/RNA Shield. To prepare these samples for our workflow, we further diluted them in our sample collection medium to 450uL before performing RNA extraction (see Methods). Figure 4 shows the results of this first clinical validation test. We have designed our workflow so that after RNA extraction, two replicate RT-qPCR plates are generated and run in parallel. Each plate must return concordant results to pass the criteria for interpreting positive or negative results. Results from one plate are shown in Figure 4 and the second plate results are in the supplementary material ( Supplementary Fig. 2) .
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