Author: Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta
Title: Cellular senescence limits acute lung injury induced by mechanical ventilation Document date: 2020_3_25
ID: ebwxryai_11
Snippet: A two-hit lung injury model, based on chlorhydric acid instillation and mechanical ventilation, was studied. Animals were anesthetized with intraperitoneal ketamine and xylazine and orotracheally intubated using a 20G catheter, through which 50μL of chlorhydric acid (0.1N, pH=1.5) were instilled. Two hours after instillation, mice were randomly assigned to receive mechanical ventilation or not. Mice were ventilated with a pressure-controlled mod.....
Document: A two-hit lung injury model, based on chlorhydric acid instillation and mechanical ventilation, was studied. Animals were anesthetized with intraperitoneal ketamine and xylazine and orotracheally intubated using a 20G catheter, through which 50μL of chlorhydric acid (0.1N, pH=1.5) were instilled. Two hours after instillation, mice were randomly assigned to receive mechanical ventilation or not. Mice were ventilated with a pressure-controlled mode (peak inspiratory pressure 17 cmH2O, PEEP 2 cmH2O, respiratory rate 100 breaths/min) for 120 minutes.
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