Author: Yanrong Li; Ying Liao; Qiaona Niu; Feng Gu; Yingjie Sun; Chunchun Meng; Lei Tan; Cuiping Song; Xusheng Qiu; Chan Ding
Title: CHOP and IRE1a-XBP1/JNK signaling promote Newcastle Disease Virus induced apoptosis and benefit virus proliferation Document date: 2018_4_12
ID: mrpj8l3q_6
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/300129 doi: bioRxiv preprint time course ( Fig. 2A) . Moreover, the pro-apoptotic BH3 only proteins BIM and PUMA, the pore forming protein BAX and BAK, kept in steady level ( Fig. 2A) . The To confirm above observation, we next adopted the transient overexpression 377 approach. A plasmid encoding the full-length human CHOP with Flag tag at the N .....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/300129 doi: bioRxiv preprint time course ( Fig. 2A) . Moreover, the pro-apoptotic BH3 only proteins BIM and PUMA, the pore forming protein BAX and BAK, kept in steady level ( Fig. 2A) . The To confirm above observation, we next adopted the transient overexpression 377 approach. A plasmid encoding the full-length human CHOP with Flag tag at the N 378 terminus was constructed. HeLa cells were transfected with the construct or vector 379 control (PXJ40F) for 24 h before being infected with NDV for 16 h. As shown in Fig. 380 2D, the successful expression of Flag-CHOP was detected with Western blotting using 381 author/funder. All rights reserved. No reuse allowed without permission.
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