Author: William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado
Title: Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice Document date: 2020_1_28
ID: ely200x3_72
Snippet: To examine how M3 muscarinic receptors influence microglia function we used the Cre-loxP strategy to generate mice expressing the human modified M3 muscarinic DREADD (hM3Dq) selectively in microglia. Specifically, we crossed the tamoxifen inducible Cre mouse line CX3CR1-CreER (Parkhurst et al., 2013) to the R26-hM3Dq/mCitrine mice (Zhu et al., 2016) to generate CX3CR1CreER(+/-)-hM3Dq(+/-), which express hM3Dq under control of the strong ubiquitou.....
Document: To examine how M3 muscarinic receptors influence microglia function we used the Cre-loxP strategy to generate mice expressing the human modified M3 muscarinic DREADD (hM3Dq) selectively in microglia. Specifically, we crossed the tamoxifen inducible Cre mouse line CX3CR1-CreER (Parkhurst et al., 2013) to the R26-hM3Dq/mCitrine mice (Zhu et al., 2016) to generate CX3CR1CreER(+/-)-hM3Dq(+/-), which express hM3Dq under control of the strong ubiquitous promoter CAG separated by the DREADD coding sequence by a floxed STOP cassette (Fig. 1A) .
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