Author: Enora Dupas; Bruno Legendre; Valérie Olivier; Françoise Poliakoff; Charles Manceau; Amandine Cunty
Title: Comparison of real-time PCR and droplet digital PCR for the detection of Xylella fastidiosa in plants Document date: 2019_3_20
ID: 0xc02kkb_8
Snippet: Addition of BSA to the reaction mix did not increase the number of droplets amplified, the 237 number of target DNA detected, nor the amplitude of the fluorescence signal. However, as it 238 increased the standard deviation between replicates, no BSA was added for the optimized 239 ddPCR protocol retained in this study (data not shown). (Table 1) . 260.....
Document: Addition of BSA to the reaction mix did not increase the number of droplets amplified, the 237 number of target DNA detected, nor the amplitude of the fluorescence signal. However, as it 238 increased the standard deviation between replicates, no BSA was added for the optimized 239 ddPCR protocol retained in this study (data not shown). (Table 1) . 260
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