Author: Shuaiyao Lu; Yuan Zhao; Wenhai Yu; Yun Yang; Jiahong Gao; Junbin Wang; Dexuan Kuang; Mengli Yang; Jing Yang; Chunxia Ma; Jingwen Xu; Haiyan Li; Siwen Zhao; Jingmei Li; Haixuan Wang; Haiting Long; Jingxian Zhou; Fangyu Luo; Kaiyun Ding; Daoju Wu; Yong Zhang; Yingliang Dong; Yuqin Liu; Yingqiu Zheng; Xiaochen Lin; Li Jiao; Huanying Zheng; Qing Dai; Qiangmin Sun; Yunzhang Hu; Changwen Ke; Hongqi Liu; Xiaozhong Peng
Title: Comparison of SARS-CoV-2 infections among 3 species of non-human primates Document date: 2020_4_12
ID: czh3zfb3_25
Snippet: Referring to the NHP model of SARS, we inoculated old world monkeys with total 4.75ml of 10 6 pfu/ml SARS-CoV-2 intratracheally (4ml), intranasally (0.5ml) and on conjunctiva (0.25ml), new world monkeys with 1.0ml intranasally. Animals were daily checked for clinical sign and body temperature. At the indicated time points in Figure 1A The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08......
Document: Referring to the NHP model of SARS, we inoculated old world monkeys with total 4.75ml of 10 6 pfu/ml SARS-CoV-2 intratracheally (4ml), intranasally (0.5ml) and on conjunctiva (0.25ml), new world monkeys with 1.0ml intranasally. Animals were daily checked for clinical sign and body temperature. At the indicated time points in Figure 1A The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031807 doi: bioRxiv preprint fluorescent-labelled antibodies for 30min in dark at room temperature. 450 ul of 1x RBC Lys solution (BD) was added to the antibody-blood mixture and incubated for 15min at room temperature to remove red blood cells, which was ready for analysis on Cytoflex machine.
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