Author: Sneha Rath; Eliza Prangley; Jesse Donovan; Kaitlin Demarest; Yigal Meir; Ned Wingreen; Alexei Korennykh
Title: Concerted 2-5A-Mediated mRNA Decay and Transcription Reprogram Protein Synthesis in dsRNA Response Document date: 2018_12_4
ID: ng5c7xai_21
Snippet: Spike-in RNA-seq indicates that the 2-5AMD-sensitive RNAs that decay (Fig. 6A , 88-89% of poly-A + RNA) account for more than 99.7% of protein synthesis (Fig. 1C , S1A). These data suggest that RNase L eliminates the most actively translating mRNAs. As much as 11-12% of the poly-A + RNA is resistant (Fig. 6A ), indicating that resistant mRNAs must be shielded from RNase L, perhaps by being in the nucleus, in phase-separated complexes or in stress.....
Document: Spike-in RNA-seq indicates that the 2-5AMD-sensitive RNAs that decay (Fig. 6A , 88-89% of poly-A + RNA) account for more than 99.7% of protein synthesis (Fig. 1C , S1A). These data suggest that RNase L eliminates the most actively translating mRNAs. As much as 11-12% of the poly-A + RNA is resistant (Fig. 6A ), indicating that resistant mRNAs must be shielded from RNase L, perhaps by being in the nucleus, in phase-separated complexes or in stress granules. The pool of RNase L-resistant poly-A + transcripts is enriched with non-coding RNAs (ncRNAs; Fig. 6A ), suggesting that translation could render mRNAs more sensitive to RNase L compared to ncRNAs. The potential dependence on translation appears to be in line with the recently proposed model for translation-dependent RNase L cleavage based on analysis of decay of exogenous RNA (Nogimori et al., 2018) .
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