Author: Clayton M. Carey; Sarah E. Apple; Zoe A. Hilbert; Michael S. Kay; Nels C. Elde
Title: Conflicts with diarrheal pathogens trigger rapid evolution of an intestinal signaling axis Document date: 2020_3_30
ID: ju826pao_23
Snippet: To generate each GC-C expressing cell line 2.5 x 10 5 293T cells were seeded into a single well of a 6 well plate. After 24 hours of growth, 50 µL concentrated lentivirus encoding GC-C linked to a GFP reporter was added to a total of 1 mL growth medium containing 8 µM polybrene (Sigma). 24 hours post transduction, cells were transferred to a T75 flask and grown for 5-7 days. Transgenic lines were established by FACS sorting the top 25% of GFP e.....
Document: To generate each GC-C expressing cell line 2.5 x 10 5 293T cells were seeded into a single well of a 6 well plate. After 24 hours of growth, 50 µL concentrated lentivirus encoding GC-C linked to a GFP reporter was added to a total of 1 mL growth medium containing 8 µM polybrene (Sigma). 24 hours post transduction, cells were transferred to a T75 flask and grown for 5-7 days. Transgenic lines were established by FACS sorting the top 25% of GFP expressing cells from each transduction.
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