Correlation of mRNA delivery timing and protein expression in lipid-based transfection

A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/607986 doi: bioRxiv preprint shows a magnification of the single-cell time courses at the beginning of the measurement illustrating the variation in the onset time points of protein expression. The cells are transfected with mRNA by adding mRNA nanocarriers using a perfusion system and incubating the complexes for one hour before the fluid is exchanged with cell growth medium (the two perfusion steps and the incubation is indicated by the arrows and the gray bar). Subsequently, we record the fluorescent protein expression time courses. Representative single-cell time courses illustrate the variation for protein expression levels (B) and eGFP expression onset times (C).