Dark Proteome of Newly Emerged SARS-CoV-2 in Comparison with Human and Bat Coronaviruses

Rajanish Giri; Taniya Bhardwaj; Meenakshi Shegane; Bhuvaneshwari R. Gehi; Prateek Kumar; Kundlik Gadhave

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.13.990598 doi: bioRxiv preprint Table 3 . Evaluation of mean predicted percentage disorder in non-structural proteins of novel SARS-CoV-2, Human SARS, and Bat CoV. Global analysis of intrinsic disorder in the replicase polyprotein 1ab Table 3 represents the PPID mean scores of 15 non-structural proteins (Nsps) derived from the Replicase polyprotein 1ab in SARS-CoV-2, Human SARS CoV, and Bat CoV. These values were obtained by combining the results from six disorder predictors (see Supplementary Table S4-S6) . Figures 17A, 17B , and 17C represent the 2D-disorder plots of the Nsps coded by ORF1ab in SARS-CoV-2, Human SARS Cov, and Bat CoV, respectively. Based on the mean PPID scores in Table 3 , Figures 17A, 17B, 17C , and taking into PPID based classification [46] , we conclude that none of the Nsps in SARS-CoV-2, Human SARS CoV, and Bat CoV are highly disordered. The highest disorder was observed for Nsp8 proteins in all three Coronaviruses. Both Nsp1 and Nsp8 are moderately disordered proteins (10% ≤ PPID ≤ 30%). We also observed that Nsp2, Nsp3, Nsp5, Nsp6, Nsp7, Nsp9, Nsp10, Nsp15, and Nsp16 have less than 10% disordered residues and hence, belong to the category of mostly ordered proteins. Other non-structural proteins, namely, Nsp4, Nsp12, Nsp13, and Nsp14 have negligible levels of disorder (PPID < 1%), which tells us that these are highly structured proteins.