Author: Prashali Bansal; Johannes Madlung; Kristina Schaaf; Boris Macek; Fulvia Bono
Title: An interaction network of RNA-binding proteins involved in Drosophila oogenesis Document date: 2020_1_9
ID: 2f9nc2to_3
Snippet: Ovaries from well-fed flies were dissected in PBS and stored at -80 ºC. For immunoprecipitation, frozen ovaries were thawed on ice in lysis buffer (50 mM Tris-HCl pH 7.5 at 4 ºC, 100 mM NaCl, 250 mM Sucrose, 0.1% NP-40 and 1 mM DTT) and pooled together in required numbers (see Table S2 ). Ovaries were mechanically homogenized in lysis buffer (320 µl/ 40 flies), supplemented with protease inhibitors (cOmplete™, EDTA-free Protease inhibitor co.....
Document: Ovaries from well-fed flies were dissected in PBS and stored at -80 ºC. For immunoprecipitation, frozen ovaries were thawed on ice in lysis buffer (50 mM Tris-HCl pH 7.5 at 4 ºC, 100 mM NaCl, 250 mM Sucrose, 0.1% NP-40 and 1 mM DTT) and pooled together in required numbers (see Table S2 ). Ovaries were mechanically homogenized in lysis buffer (320 µl/ 40 flies), supplemented with protease inhibitors (cOmplete™, EDTA-free Protease inhibitor cocktail, Roche), using tissue homogenizer with a glass pestle.
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