Author: Prashali Bansal; Johannes Madlung; Kristina Schaaf; Boris Macek; Fulvia Bono
Title: An interaction network of RNA-binding proteins involved in Drosophila oogenesis Document date: 2020_1_9
ID: 2f9nc2to_4
Snippet: Lysates were cleared by centrifugation at 21,000g for 20 min at 4 ºC and 5 µl/ml of RNase A/T1 (Thermo Fisher Scientific) was added to the supernatants. After incubation at 4 ºC for 30 min, lysates were cleared again and 30-60 µl of GFP-TRAP MA beads (Chromotek) were added. The mixtures were incubated for 1 hour at 4 ºC in rotation. Beads were washed with lysis buffer and proteins were eluted as described above......
Document: Lysates were cleared by centrifugation at 21,000g for 20 min at 4 ºC and 5 µl/ml of RNase A/T1 (Thermo Fisher Scientific) was added to the supernatants. After incubation at 4 ºC for 30 min, lysates were cleared again and 30-60 µl of GFP-TRAP MA beads (Chromotek) were added. The mixtures were incubated for 1 hour at 4 ºC in rotation. Beads were washed with lysis buffer and proteins were eluted as described above.
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