Author: Prashali Bansal; Johannes Madlung; Kristina Schaaf; Boris Macek; Fulvia Bono
Title: An interaction network of RNA-binding proteins involved in Drosophila oogenesis Document date: 2020_1_9
ID: 2f9nc2to_40
Snippet: To validate the results of our IP-MS analysis, we co-expressed bait-candidate pairs in cultured mammalian HEK293 cells. This system can be effectively used to study direct interactions of Drosophila proteins because it reduces the likelihood of endogenous proteins mediating indirect associations. For validation, we selected significantly enriched candidates identified in the label-free MS analysis. Additionally, we also considered functionally re.....
Document: To validate the results of our IP-MS analysis, we co-expressed bait-candidate pairs in cultured mammalian HEK293 cells. This system can be effectively used to study direct interactions of Drosophila proteins because it reduces the likelihood of endogenous proteins mediating indirect associations. For validation, we selected significantly enriched candidates identified in the label-free MS analysis. Additionally, we also considered functionally relevant partners, enriched with a >2 times fold change but excluded by statistical filtering. For
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