Author: Armin Ensser; Klaus Ueberla
Title: Determination of daily reproduction numbers of SARS-CoV2 based on death cases suggests more rapid initial spread in Italy and the United States Document date: 2020_3_31
ID: 4izymiy4_22
Snippet: Next, we assayed acutely infected CEM-SS-R5 cells that had been cultured for 12 h after spinoculation (Fig. 5C) . Again, we found that one-way amplification with the gag primer resulted in a small increase in signal above no preamplification. In this acutely infected sample, a 2.7 log increase in signal over no preamplification was observed with bidirectional Alu-gag amplification. This relatively modest shift is due to the predominance of uninte.....
Document: Next, we assayed acutely infected CEM-SS-R5 cells that had been cultured for 12 h after spinoculation (Fig. 5C) . Again, we found that one-way amplification with the gag primer resulted in a small increase in signal above no preamplification. In this acutely infected sample, a 2.7 log increase in signal over no preamplification was observed with bidirectional Alu-gag amplification. This relatively modest shift is due to the predominance of unintegrated HIV-1 DNA at this early time point. Specifically, this sample had a mean of 2.24 Ï® 0.06 copies of viral HIV-1 LTR DNA per cell, but only 0.11 Ï® 0.04 proviruses per cell (n Ï 3).
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