Selected article for: "LOD detection and low concentration"
Author: Gun-Soo Park; Keunbon Ku; Seung-Hwa Beak; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Jin-Soo Maeng
Title: Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
  • Document date: 2020_3_12
    • ID: 1qn5y4gc_26
    Snippet: The Limit of Detection (LoD) of optimized RT-LAMP assays were assessed through 5 to 1000 RNA copies/reaction in triplicate. Among four primer sets subjected to reaction optimization, "S_1-2-2" and "N_21" sets showed relatively poor sensitivity. For "Nsp3_1-61" and "Nsp3_2-24" primer sets, we additionally evaluated LoD with 2-fold SuperScript IV Reverse Transcriptase (20U/reaction). LoD and Tt_av were improved by increasing reverse transcriptase a.....
    Document: The Limit of Detection (LoD) of optimized RT-LAMP assays were assessed through 5 to 1000 RNA copies/reaction in triplicate. Among four primer sets subjected to reaction optimization, "S_1-2-2" and "N_21" sets showed relatively poor sensitivity. For "Nsp3_1-61" and "Nsp3_2-24" primer sets, we additionally evaluated LoD with 2-fold SuperScript IV Reverse Transcriptase (20U/reaction). LoD and Tt_av were improved by increasing reverse transcriptase amount. (Table 2, Supplementary Figure 2) As a result, both "Nsp3_1-61" and "Nsp3_2-24" RT-LAMP assays could detect as low RNA concentration as 100 copies per reaction.

    Search related documents:
    Co phrase search for related documents
    • low RNA concentration and reverse transcriptase: 1, 2
    • low RNA concentration and RNA concentration: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • low RNA concentration detect and reverse transcriptase: 1
    • low RNA concentration detect and RNA concentration: 1, 2
    • optimization reaction and primer set: 1
    • optimization reaction and reverse transcriptase: 1
    • optimization reaction and RNA concentration: 1
    • primer set and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8
    • primer set and RNA concentration: 1, 2, 3
    • primer set and RNA copy: 1, 2, 3, 4, 5
    • primer set and RT LAMP assay: 1, 2, 3, 4, 5, 6, 7, 8
    • reverse transcriptase and RNA concentration: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • reverse transcriptase and RNA copy: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • reverse transcriptase and RT LAMP assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
    • reverse transcriptase and triplicate reaction: 1
    • RNA copy and RT LAMP assay: 1, 2, 3, 4, 5, 6