Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone

Sanchita Bhadra; Timothy E. Riedel; Miguel A. Saldaña; Shivanand Hegde; Nicole Pederson; Grant L. Hughes; Andrew D. Ellington

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/291849 doi: bioRxiv preprint albopictus and Cu. quinquefasciatus mosquitoes, which are naturally infected with wAlbB and 307 wPip Wolbachia, respectively, but remained negative in the presence of unrelated Wolbachia 308 wMel and uninfected mosquitoes (Fig 2 and S4 Fig) . These results indicate that the Wolbachia wsp and Ae. aegypti coi LAMP-OSD assays are able 325 to specifically amplify and signal the presence of their target DNA directly from crudely crushed 326 All rights reserved. No reuse allowed without permission.