Selected article for: "Ct value and standard curve"
Author: Jonathon A. Siva-Jothy; Pedro F. Vale
Title: Dissecting genetic and sex-specific host heterogeneity in pathogen transmission potential
  • Document date: 2019_8_14
    • ID: 0v5q4kp9_5
    Snippet: (1-10 -12 ) of DCV cDNA. Our detection threshold was calculated for each plate using 202 the point at which two samples on our standard curve gave the same Ct value. The 203 point of redundancy in a standard curve was taken to be equivalent to 0 viral 204 particles. Due to our detection protocol measuring viral copies of RNA, we cannot 205 comment on the viability of any detected virus. We transformed our measurements of 206 viral RNA transformed.....
    Document: (1-10 -12 ) of DCV cDNA. Our detection threshold was calculated for each plate using 202 the point at which two samples on our standard curve gave the same Ct value. The 203 point of redundancy in a standard curve was taken to be equivalent to 0 viral 204 particles. Due to our detection protocol measuring viral copies of RNA, we cannot 205 comment on the viability of any detected virus. We transformed our measurements of 206 viral RNA transformed in order for them to represent the amount of virus growing 207 inside a whole fly rather than the amount in the qPCR well sample. To account for 208

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