Author: Ze Chen; Xiaofeng Xu; Xiaojun Yang; Weihao Dou; Xiufeng Jin; Haishuo Ji; Guangyuan Liu; Jianxun Luo; Hong Yin; Gao Shan
Title: DNA segment of African Swine Fever Virus first detected in hard ticks from sheep and bovine Document date: 2018_12_3
ID: 36rq4rmg_4
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/485060 doi: bioRxiv preprint nuttalli adults (replicate), D. nuttalli larvae and D. nuttalli nymphs (Materials and Methods). VirusDetect 86 reported the existence of ASFV in all four D. nuttalli samples, but not in A. testudinarium adults. Aligning 87 the cleaned reads to the ASFV reference genome (GenBank: AY261365.1), the mapping rates of four .....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/485060 doi: bioRxiv preprint nuttalli adults (replicate), D. nuttalli larvae and D. nuttalli nymphs (Materials and Methods). VirusDetect 86 reported the existence of ASFV in all four D. nuttalli samples, but not in A. testudinarium adults. Aligning 87 the cleaned reads to the ASFV reference genome (GenBank: AY261365.1), the mapping rates of four D. 88 nuttalli samples reached 0.06% (15,585/25,194,632) , 0.06% (23,330/37,888,277) , 0.08% 89 (10,241/12,302,335) and 0.08% (12,807/15,077,054), which were significantly higher than the mapping rate 90 0.01% (905/13,496,191) Sanger sequencing of it failed due to low DNA concentration. To confirm these results, we used PCR to 105 author/funder. All rights reserved. No reuse allowed without permission.
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