Domestication reprogrammed the budding yeast life cycle

Matteo De Chiara; Benjamin Barré; Karl Persson; Amadi Onyetuga Chioma; Agurtzane Irizar; Joseph Schacherer; Jonas Warringer; Gianni Liti

. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.08.939314 doi: bioRxiv preprint Supplementary Figure 4 . Loss-of-function variants in SPO12 and SPO13 trigger dyadsonly spore production. a, Sporulation (asci production, y-axis) after 3 days in standard experimental sporulation environment (KAc) and the percentage of produced asci that are dyads (x-axis), after 24 h (left panel) and 72 h (right panel). Nine strains, belonging to two clusters (indicated in green and orange) efficiently enter sporulation but interrupt meiosis cycle after meiosis I, only producing dyads. b, Micrograph of asci produced in strain BNL, after 72 h of sporulation, with staining of the spore wall (blue). Only dyads are produced. c, Schematic representation of amino acid sequences for SPO12 and SPO13 showing position of homozygous loss-of-function variants that trigger dyads-only production during sporulation.