Selected article for: "CDC assay and double quencher"
Author: Yosuke Hirotsu; Hitoshi Mochizuki; Masao Omata
Title: Double-Quencher Probes Improved the Detection Sensitivity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by One-Step RT-PCR
  • Document date: 2020_3_20
    • ID: 2gokv7id_25
    Snippet: The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037903 doi: medRxiv preprint (Figure 3) . Similarly, the signal in the N2 site of NIID was around 125×10 4 to 200×10 4 , and that of YCH was 75×10 4 to 100×10 4 . Results suggested that double-quencher probes improved the detection sensitivity of SARS-CoV-2 using one-step real-time RT-PCR owing to the reduction of background signal. They showed that ORF1ab is best a.....
    Document: The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037903 doi: medRxiv preprint (Figure 3) . Similarly, the signal in the N2 site of NIID was around 125×10 4 to 200×10 4 , and that of YCH was 75×10 4 to 100×10 4 . Results suggested that double-quencher probes improved the detection sensitivity of SARS-CoV-2 using one-step real-time RT-PCR owing to the reduction of background signal. They showed that ORF1ab is best according to China. In the N gene, CDC-N2 (USA), CDC-N3 (USA), and NIID-N2 (Japan) sets should be selected for sensitive and reliable laboratory confirmation of SARS-CoV-2. We have also observed that the CDC-N2 (USA) and NIID-N2 (Japan) assay could detect low number of DNA positive control.

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